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2009 Oct-Nov;25(9-10):577-81
Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1/ZEB1 axis
lactis strains transformed with the different plasmids used in this work were routinely grown at 30 C in M-17 liquid medium (Millipore - Merck KGaA, Darmstadt, Germany) supplemented with 0.5% glucose (Sigma-Aldrich - Merck KGaA, Darmstadt, Germany) (GM-17 medium) without agitation, or in plates containing the same medium solidified with 1.5% agar (Carlo Erba, Cornaredo (MI), Italy)
At the same time, the radius of gyration (Rg) of the Ld3MST-Rutin complex converged over time, and the solvent accessible surface area (SASA, ~44 nm 2 ) remained stable, indicating a compactness and a more stable binding of the complex
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